Abberior FLIP

Abberior FLIP 565 is a photoswitchable dye that belongs to the photosensitive spiroamide compounds. Upon photoswitching with UV light at 360 - 375 nm they form transiently fluorescent species (excitation maximum: 565nm; emission maximum: 580 nm). Shortly after this Abberior FLIP 565 molecules return to the nonfluorescent state via thermal relaxation. The dye is performing very well in PALM and STORM microscopy e.g. a Nikon N-STORM microscope.

 

 

Selected references:

 

 

Roubinet B et al. "Photoactivatable Rhodamine Spiroamides and Diazoketones Decorated with "Universal Hydrophilizer" or Hydroxyl Groups" The Journal of Organic Chemistry 83(12):6466-6476, DOI: 10.1021/acs.joc.8b00756 (2018)

Bossi M et al. "Multicolor Far-Field Fluorescence Nanoscopy through Isolated Detection of Distinct Molecular Species" Nano Letters 8(8):2463-8, DOI: 10.1021/nl801471d (2008)

Fölling J et al. "Photochromic Rhodamines Provide Nanoscopy with Optical Sectioning" Angewandte Chemie International Edition 46(33):6266-70, DOI: 10.1002/anie.200702167 (2007)