Abberior 595 STED

适合染剂发光光谱在绿色光谱区域的应用, 可搭配升级775 nm 激光, 做到多色超分辨荧光成像.

Abberior 595 STED ( Infinity system ) 先进的共聚焦超分辨纳米成像系统, 配置在红外光谱的脉冲式 595 nm STED 压制激光, 可做到单次扫描及直接呈现纯光学解析优于 25 nm (不需影像的虚拟后制处理) 的双色荧光超分辨率成像. 3D 解析优于 75x75x75 nm

适宜生物医学与光电物理研究

可搭载前瞻技术 RESCue STED, DyMIN STED, MINFIELD 的智能型光照调控设计, 建构高速活细胞多色荧光超分辨成像应用 (LIVE-CELL STED).

详细介绍

Abberior 595 STED
先进的共聚焦超分辨纳米成像系统, 配置595 nm STED 压制激光, 实现多色超分辨率影像, 适合GFP, YFP 荧光蛋白, 或者, 有机染剂, 如Abberior STAR 440SX or STAR 488等成像应用.

范例 :

双色 STED 荧光应用 (595 nm STED), 可选用 STAR 440XP, STAR 488, 可再配合 SiR 染剂.

2-color STED images with the Abberior STAR 440 SXP and Abberior STAR 488

Labels are Abberior STAR 488 (peroxisomal membrane protein PMP70) and STAR 440 SXP (Tubulin)
Abberior STAR 440 SXP is the optimal long-stokes partner to enable 2-color STED with one laser wavelength at ~590nm.
The dye pair is tested and verified for the Abberior-Instruments 2-color STED 595 microscope operating a 595 nm pulsed STED laser

Impressions

G. Vicidomini, G. Moneron, K. Y. Han, V. Westphal, H. Ta, M. Reuss, J. Engelhardt, C. Eggeling, S. W. Hell, "Sharper low-power STED nanoscopy by time gating", Nature Meth. 8, 571 - 573. (2011)
S. Berning, K. I. Willig, H. Steffens, P. Dibaj, S. W. Hell, "Nanoscopy in a Living Mouse Brain", Science 335, 551 (2012)
B. Harke, J. Keller, C. K. Ullal, V. Westphal, A. Schönle, S. W. Hell, "Resolution scaling in STED microscopy", Opt. Express 16, 4154-4162 (2008)
B. R. Rankin, G. Moneron, C. A. Wurm, J. C. Nelson, A. Walter, D. Schwarzer, J. Schroeder, D. A. Colo´n-Ramos, S. W. Hell, "Nanoscopy in a Living Multicellular Organism Expressing GFP", Biophysical J. Volume 100, L63–L65 (2011)
J. Tønnesen, F. Nadrigny, K. I. Willig, R. Wedlich-Söldner, V. Nägerl, "Two-Color STED Microscopy of Living Synapses Using A Single Laser-Beam Pair", Biophys J., 101(10): 2545–2552 (2011)
B. Hein, K. I. Willig, S. W. Hell, "Stimulated emission depletion (STED) nanoscopy of a fluorescent protein-labeled organelle inside a living cell", PNAS 105, 14271-14276 (2008)
K. I. Willig, S. O. Rizzoli, V. Westphal, R. Jahn, S. W. Hell, "STED microscopy reveals that synaptotagmin remains clustered after synaptic vesicle exocytosis", Nature, Vol. 440|13 (2006)