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显微镜用特殊温控(高温与低温)台

将细胞和生物组织保持在受控环境中是了解其体内行为的关键。

英国 LINKAM 公司, 有别于一般活细胞培养的仿生环境控制, 其设计的温度差控制系统,  被广泛用于从低温保存到 -195°C 的 Correlative Light and Electron Microscopy (CLEM), 从高温和高压下的 Archae 研究.   
 
LINKAM 的功能, 可以控制相对湿度从 5％ 到 90％。
 
产品广泛应用 从生物样本到材料科学, 温控范围可从 -195°C 到 +1500°C.

详细介绍

 

THMS600

Temperature and environmental control from < -195°C to 600°C with XY sample manipulators

LNP system with Dewar, THMS600 and Imaging Station, T96 and Linkpad

The LNP96 cooling system is uniquely designed by Linkam. The precise control of liquid nitrogen flow enables specific stages to be controlled at linear cooling rates as fast as 100°C/min or as slow as 0.01°C/min. The unique design of the LNP96 reduces noise and vibration and is automatically controlled by the T96 controller. A cooling system consists of the control unit housing the pumps and a Dewar. The LNP96 can be added as an option to several Linkam systems to allow cooling down to < -195°C.

THMS600 Specifications

  • Temperature range < -195°C to 600°C (cooling option required)

  • Up to 150°C/min heating, 100°C/min cooling (using LNP96 cooling pump)

  • Temperature stability <0.1°C

  • 16 mm XY sample manipulation

  • Sample area 22 mm diameter

  • Quick-release gas valves for atmospheric control

  • 100ohm platinum resistor sensor. 1/10th Din Class B to 0.1°C

  • Light aperture - 2.0mm Ø

  • Silver heating block for high thermal conductivity

  • Direct injection of the coolant into the silver block

  • Single ultra thin lid window - 0.5mm

  • Objective lens working distance 4.8mm

  • Condenser lens minimum working distance 12.5mm

  • Range of condenser extension lenses available

  • Can be used with all microscope techniques

  • The stage body can be cooled with a water cooling pump for high temperature work (>300°C)

  • Suitable for confocal, Laser Raman and X-ray

  • Sample side loading without removing the stage lid

 

 

RH95 相对湿度控制器

旨在为Linkam的温度范围提供环境样品控制。它以紧凑，独立的包装提供精确控制，而无需外部干燥空气供应。
RH95可以将温度控制与阶段内相对湿度的控制相结合。湿度会对材料的性能产生重大影响。其中包括对样品的机械性能变化和化学变化（如产生多晶型物）的表面修饰作用。 RH95具有广泛的应用，包括测试和表征新型光伏材料，研究新型电池技术以及表征药物。

 

与其他湿度系统不同，不需要昂贵的干燥空气供应。通过专门设计的自动循环干燥剂系统干燥环境空气。该系统可以一次控制几个月的湿度。

 

对于存在氧化问题的应用，RH95可以提供可选的惰性气体调节器，该调节器允许使用氮气作为载气

 

 

 

 

 

LINK Specification

  • LINK Core

    • Easy to use and intuitive interface

    • Full control of all Linkam stages

    • Manual and automated control of temperature and other parameters

    • Real time chart of temperature and other measured parameters

    • Row by row control of vacuum, humidity, tensile force and shear modes controllable and synchronised with temperature

    • Parameters of connected Linkam sensors displayed and stored - e.g. vacuum, humidity, force, XY position (dependent on connected stage type)

    • Trigger I/O for synchronisation with other equipment

    • Triggers recorded and plotted row by row

    • A profile can contain up to 100 ramps

    • Full five point temperature calibration

    • Data export to standard CSV format for further analysis

    • USB 2.0 to T96

    • Native 64 bit application

Optional Modules

  • LINK Imaging System

    • Automated image capture with ramp

    • Distance calibration (Reticule supplied)

    • Image capture synchronised with temperature

    • Images stamped with temperature, time and other measured parameters

    • Image export - multiple image types supported

    • Images linked to temperature chart for easy review

    • Image capture at up to 30fps

    • Gallery view

    • Movie generator

  • LINK 21CFR11

    • 21 CFR 11 compliance module for LINK

    • Access control via Windows logon

    • Supports user level management

    • Audit trail

    • NB Users need to be a member of a Domain Group

  • LINK Extended Measurements

    • Multiple image measurement tools for distance, angle and area

    • Fully calibrated measurements

    • Annotation tools

    • Measurements and annotations can be burned into the image for quick and easy analysis

  • LINK TASC

    • Thermal Analysis by Surface Characterisation. Click here for more details on this technique

 

电子显微镜（EM）可实现高分辨率的结构信息。 但是，由于染色和样品制备过程的局限性，它只能对生物学和化学过程提供有限的洞察力。 另一方面，荧光显微镜是检测活细胞内生物学，化学和遗传过程及事件的非常灵敏的方法。
Cryo-CLEM将所有内容融合在一起：这是一种新兴技术，通过使用两种技术对同一样品位置进行成像并迭加补充信息，从而结合了荧光和EM的优势。

 

LINKAM 提供 CLEM 样本制备解决方案 :

冷冻条件下玻璃化样品的成像
 

为了使生物样品与EM中的真空条件兼容并保留结构细节，将样品嵌入玻璃化的“玻璃状”冰中并需要保持在-140°C以下。 由于冰晶会立即形成并污染样品，因此必须避免与空气中的水分接触。 在低温条件下，荧光信号的结构细节得以保留，光漂白显著减少。